Pham Mitochondrial Physiology Laboratory
The Pham Laboratory at the University of Miami is focused on measuring and improving mitochondrial health in models of optic neuropathy using a multi-modal approach. We develop murine models to enable high-resolution imaging analysis for quantifying mitochondrial clearance and dynamics in primary tissues, organotypic cultures, and retinal explants. We are exploring innovative methodologies to edit mitochondrial DNA using CRISPR-based technology.
Recent Highlights
Reliability of Visual Field Testing in a Telehealth Setting Using a Head-Mounted Device: A Pilot Study
Demonstrating the reliability of head-mounted visual field testing for telehealth, offering a promising alternative to traditional in-clinic perimetry.
Establishing induced pluripotent stem cell lines from two dominant optic atrophy patients with distinct OPA1 mutations and clinical pathologies
Establishing iPSC lines from two dominant optic atrophy patients with different OPA1 mutations, providing valuable models for disease research and therapeutic development.
Development of a CRISPR/Cas9 System for Mitochondrial Diseases
Presenting a CRISPR/Cas9 system tailored for mitochondrial diseases, paving the way for targeted genetic therapies.